GISH Analysis of the Introgression of the B Subgenome Genetic Material of Wild Allotetraploid Species Solanum stoloniferum into Backcrossing Progenies with Potato

Wild relatives of cultivated potato are used in breeding to increase the genetic diversity Solanum tuberosum (AAAA genome) varieties. Mexican allotetraploid species stoloniferum (AABB was for extreme resistance viruses and late blight. In this study, genomic situ hybridization (GISH) visualization introgression material B subgenome S. into genome backcross hybrids. The fertile hexaploid hybrid had 48 chromosomes A 24 subgenome. Plants BC1 generation were pentaploid having AAAAB constitution three selected BC2 hybrids aneuploid, containing one six genome. inherited generations as single rare cases recombinant chromosomes. GISH showed that chromosome pairing predominantly intragenomic. Most remained univalents Rare homeologous A/B detected all analyzed obtained data indicate able recombine with